The two schools haven't played in football since 2010, when the then No. 8-ranked Gators won 38-14.
The Gators have never been to Tampa, but will make their first trip in 2023.
The three scheduled games are Sept. 17, 2022 in Gainesville, Sept. 9, 2023 in Tampa and Sept. 6, 2025 in Gainesville.
"We are very excited for our program and our fans to add three games against the University of Florida to our schedule," Harlan said. "I would like to thank Coach Strong for his desire to play a challenging non-conference schedule and the leadership at Florida for their partnership in making this happen. We are committed to scheduling opponents that our fans are excited to see the Bulls compete against and Florida certainly ranks very high on that list."
Bulls head coach Charlie Strong began his coaching career at UF.
USF's previously scheduled matchup with FAU on Sept. 17, 2022 in Tampa will be moved to a future date. A 2023 return game vs. San Jose State in Tampa, previously scheduled for Sept. 9, has been moved to a Sept. 16, 2023 kickoff in Tampa.